Plasmid

Part:BBa_I11008:Design

Designed by: Lutz and Bujard   Group: Antiquity   (2004-08-18)


pUHS*2Plac/ara-1 plasmid (MCS 1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 500
    Illegal EcoRI site found at 569
    Illegal SpeI site found at 2254
    Illegal PstI site found at 575
    Illegal PstI site found at 3686
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 500
    Illegal EcoRI site found at 569
    Illegal SpeI site found at 2254
    Illegal PstI site found at 575
    Illegal PstI site found at 3686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 500
    Illegal EcoRI site found at 569
    Illegal BglII site found at 2272
    Illegal BglII site found at 3899
    Illegal BamHI site found at 425
    Illegal BamHI site found at 587
    Illegal XhoI site found at 392
    Illegal XhoI site found at 536
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 500
    Illegal EcoRI site found at 569
    Illegal SpeI site found at 2254
    Illegal PstI site found at 575
    Illegal PstI site found at 3686
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 500
    Illegal EcoRI site found at 569
    Illegal SpeI site found at 2254
    Illegal PstI site found at 575
    Illegal PstI site found at 3686
    Illegal NgoMIV site found at 802
    Illegal NgoMIV site found at 3235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3175
    Illegal SapI site found at 3385


Design Notes

Source

References